.. in “International Journal of Applied Pharmaceutics” which is classified within the containers (Scopus and Clarivate) and in (second) quarter.. The research included a study of the effect of 50 Hz frequency frequency (1 MT intensity) on the osteo genic differentiation of rat bone marrow stem cells in the laboratory to demonstrate the broad effect resulting from the use of The research was divided into three groups of rat bone marrow and treated with different conditions and media, and then the activity and QRT-PCR were performed Results: The electromagnetic fields group showed weaker positive spots for ALP and alizarin red compared to the positive control group. The Runx2 and Ocn genes Expression levels decreased significantly compared to the negative control at 14 and 21 days of exposure to electromagnetic fields, respectively. After 14 and 21 d Exposure, Runx2 and Ocn gene expression was significantly lower in the EMF group than in the positive control group Conclusion: SEMF (1 MT, 50 Hz, 30 min/day) can delay osteogenesis and reduce osteogenic differentiation of rBMSCs